Meat ID™ Halal Identification (Kandungan Babi)

Meat ID™ Halal is a kit for the fast and reliable identification of pork and donkey in meat and other foods by qPCR.

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Description

The identification of different meats, especially in minced meat products is a serious task in food safety and from and ethical perspective, especially for Muslims. Authentication of forbidden or none declared ingredients such as pork or donkey meat is essential to ensure confidence in the supply chain and regulatory compliance.

Meat ID™ Halal is available for rapid and reliable analysis from various matrices including raw, or even highly processed and cooked meat products where the DNA may be significantly degraded. It is possible to identify relevant species down to a threshold level of 0.5% with a semi-quantitative result.

INDICATION
The identification of different meat types, especially in minced meat products is a serious task in food safety and from an ethical perspective, especially for Muslims. Authentication of forbidden or non-declared ingredients such as pork or substandard meat is essential to ensure confidence in the supply chain and the regulatory compliance.
Meat ID™ Halal is a kit for the fast and reliable identification of pork and donkey contaminations in meat and other foods by qPCR, and is provided as lyophilized ready to use master mix. Meat ID™
Halal is available for rapid and reliable analysis from various matrices including raw, or even highly processed and cooked meat products where the DNA may be significantly degraded. It is possible to identify pork and donkey down to a threshold level of 0.5% with a semi-quantitative result.

TEST PRINCIPLE
The assay is based on the TaqMan® principle and relies on the 5’→ 3’ exonuclease activity of Taq
polymerase as well as the dual labeling of the probes with fluorescents and quenchers. During
PCR the Taq polymerase cleaves and removes annealed probes releasing the previously quenched
fluorescent signal. TaqMan® based qPCR is compatible with both FAM™ and HEX™ labeled probes.
The target sequence is a mitochondrial multi-copy gene (cytochrome b). Therefore, occurrence of
even small amounts of DNA (down to 1 pg/PCR-reaction) could lead to positive results. Primer sets
are specific to the animal species tested.

False-negative results caused by PCR inhibition and/or DNA extraction issues will be reliably identified by means of the Internal Control DNA. The Internal Control DNA is either added directly to the PCR master mix to function as a PCR amplification control, or is alternatively added to the original sample prior to DNA extraction. By adding the Internal Control DNA directly to the sample, both DNA extraction and qPCR amplification are validated. The internal control amplification is detected at 560 nm (HEX channel), whereas the species-specific amplification is detected at 520 nm (FAM channel).

 

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